منابع مشابه
Laser Scanning Confocal Microscopy
Laser scanning confocal microscopy has become an invaluable tool for a wide range of investigations in the biological and medical sciences for imaging thin optical sections in living and fixed specimens ranging in thickness up to 100 micrometers. Modern instruments are equipped with 3-5 laser systems controlled by high-speed acousto-optic tunable filters (AOTFs), which allow very precise regula...
متن کاملConfocal laser scanning microscopy.
Many technological advancements of the past decade have contributed to improvements in the photon efficiency of the confocal laser scanning microscope (CLSM). The resolution of images from the new generation of CLSMs is approaching that achieved by the microscope itself because of continued development in digital imaging methods, laser technology and the availability of brighter and more photos...
متن کاملScanning and Image Reconstruction Techniques in Confocal Laser Scanning Microscopy
Taking advantage of the laser scanning methodology, confocal microscopy has been widely applied in cutting-edge biological research for its three-dimensional, high-resolution imaging capability (Pawley 1995). The principles of confocal were put forward by Marvin Minsky in 1955 (Minsky 1988), yet the experimental demonstration was not accomplished until 20 years later by Cremer brother (Cremer, ...
متن کاملLaser scanning laser diode photoacoustic microscopy system
The development of low-cost and fast photoacoustic microscopy systems enhances the clinical applicability of photoacoustic imaging systems. To this end, we present a laser scanning laser diode-based photoacoustic microscopy system. In this system, a 905 nm, 325 W maximum output peak power pulsed laser diode with 50 ns pulsewidth is utilized as the light source. A combination of aspheric and cyl...
متن کاملCombining Atomic Force Microscopy and Laser Scanning Confocal Microscopy
Introduction The visualisation of sample elements using various forms of microscopy relies not only on magnification but also contrast. As such, differing forms of microscopy offer different information about a sample. Laser scanning confocal microscopy (LSCM) provides information about the 3D location of a particular, labelled component within a sample and has the additional advantage of exclu...
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ژورنال
عنوان ژورنال: Biomedical Optics Express
سال: 2014
ISSN: 2156-7085,2156-7085
DOI: 10.1364/boe.5.001588